NMNAT3 Rabbit pAb (20 μl)

NMNAT3 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14465
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Clonality: Polyclonal antibody    
Gene Name: nicotinamide nucleotide adenylyltransferase 3
Gene Symbol: NMNAT3
Synonyms: PNAT3; FKSG76; PNAT-3; NMNAT3
Gene ID: 349565
UniProt ID: Q96T66
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NMNAT3 (NP_835471.1).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 2.17 mg/ml
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-NMNAT3 antibody




Background

This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.




Images

LRP8 antibodyWestern blot analysis of various lysates using NMNAT3 Rabbit pAb (A14465) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using NMNAT3 Rabbit pAb (A14465) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using NMNAT3 Rabbit pAb (A14465) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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