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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | 5-oxoprolinase, ATP-hydrolysing |
| Gene Symbol: | OPLAH |
| Synonyms: | OPLA; OPLAHD; 5-Opase; OPLAH |
| Gene ID: | 26873 |
| UniProt ID: | O14841 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.62 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-OPLAH antibody |
Background
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Images
 | Western blot analysis of various lysates using OPLAH Rabbit pAb (A12032) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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