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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | RNA binding motif protein 25 |
| Gene Symbol: | RBM25 |
| Synonyms: | S164; NET52; RNPC7; Snu71; RED120; fSAP94; RBM25 |
| Gene ID: | 58517 |
| UniProt ID: | P49756 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1). |
| Dilution: | WB 1:200-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.87 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RBM25 antibody |
Background
Enables mRNA binding activity. Involved in regulation of alternative mRNA splicing, via spliceosome and regulation of apoptotic process. Located in nuclear speck.
Images
 | Western blot analysis of various lysates using RBM25 Rabbit pAb (A15496) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U-2 OS cells using RBM25 Rabbit pAb (A15496) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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