| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Protein S100A4 |
Synonyms: | 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, S100 calcium binding protein A4 |
Immunogen: | Recombinant protein corresponding to Mouse S100A4 |
Uniprot ID: | P26447, P07091, P05942 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Secreted, Nucleus |
Predicted MW. / Observed MW. | 12 kDa / 12 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 2000 | HeLa, NIH/3T3, A549, Hep G2, C2C12, PC-12, C6 |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 1600 | spleen, colon, kidney, endometrial cancer |
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
Images
| Western blot analysis of S100A4 (GB15398) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat colon using S100A4 (GB15398) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using S100A4 (GB15398) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded mouse colon using S100A4 (GB15398) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded human kidney using S100A4 (GB15398) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded human endometrial cancer using S100A4 (GB15398) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded rat spleen using S100A4 (GB15398) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |