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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SUMO1 activating enzyme subunit 1 |
| Gene Symbol: | SAE1 |
| Synonyms: | AOS1; SUA1; UBLE1A; HSPC140; SAE1 |
| Gene ID: | 10055 |
| UniProt ID: | Q9UBE0 |
| Clone ID: | 0L2N2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SAE1 (Q9UBE0). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.6 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SAE1 antibody |
Background
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Images
 | Western blot analysis of various lysates using SAE1 Rabbit mAb (A0891) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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