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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ubiquitin like modifier activating enzyme 5 |
| Gene Symbol: | UBA5 |
| Synonyms: | DEE44; EIEE44; SCAR24; THIFP1; UBE1DC1; UBA5 |
| Gene ID: | 79876 |
| UniProt ID: | Q9GZZ9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UBA5 (NP_079094.1). |
| Dilution: | WB 1:200-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.35 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-UBA5 antibody |
Background
This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.
Images
 | Western blot analysis of various lysates using UBA5 Rabbit pAb (A15514) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat liver using UBA5 Rabbit pAb (A15514) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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