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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | YTH N6-methyladenosine RNA binding protein F1 |
| Gene Symbol: | YTHDF1 |
| Synonyms: | DF1; C20orf21; YTHDF1 |
| Gene ID: | 54915 |
| UniProt ID: | Q9BYJ9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2). |
| Dilution: | WB 1:100-1:500; IF/IC 1:50-1:200; IP,1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.92 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-YTHDF1 antibody |
Background
Enables N6-methyladenosine-containing RNA binding activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule.
Images
 | Western blot analysis of various lysates, using YTHDF1 Rabbit pAb (A13260) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using YTHDF1 Rabbit pAb (A13260) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using YTHDF1 Rabbit pAb (A13260) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using YTHDF1 Rabbit pAb (A13260) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using YTHDF1 Rabbit pAb (A13260) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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