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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | zinc finger E-box binding homeobox 1 |
| Gene Symbol: | ZEB1 |
| Synonyms: | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; ZEB1 |
| Gene ID: | 6935 |
| UniProt ID: | P37275 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 490-710 of human ZEB1 (NP_110378.3). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:100-1:200 |
| Purification Method: | Affinity purification |
| Buffer: | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ZEB1 antibody |
Background
This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
Images
 | Western blot analysis of lysates from Jurkat cells, using ZEB1 Rabbit pAb (A1500) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using ZEB1 Rabbit pAb (A1500) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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