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Recombinant Anti-beta Actin antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Actin, cytoplasmic 1 |
Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Conjugate | HRP |
Purity: | Affinity purification |
Uniprot ID: | P60710 |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1:1000-1:3000 | HeLa, HepG2, brain, etc. |
Incubation condition | After blocking, incubate the membrane with HRP conjugated anti-beta Actin antibody (ZB15001-HRP) for 2-3 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
| Westerin blot analysis of beta actin (ZB15001-HRP) Sample: Protein treated by RIPA Lysis Buffer (G2002). |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |