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Recombinant Anti-beta Actin Rabbit mAb (100 μl)
| Reactivity: | H,R,M & H,R,M & H,R,M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal ) |
| Gene Name: | Actin, cytoplasmic 1 |
Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
Immunogen: | KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
Uniprot ID: | P60710 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
| reactivity （External validation） | Pelodiscus sinensis |
Product Usage Information
WB | Human, Mouse, Rat | 1: 3000-1: 5000 | HeLa, Jurkat, MCF-7, PC-3, HepG2 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, colon cancer, lung, lung cancer |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, HeLa |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
| WB analysis of beta Actin(Rab) (GB15003). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 5000, RT, 1h. |
| Immunohistochemistry of paraffin embedded mouse spleen using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human colon cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human lung cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded rat lung using beta-Actin (GB15003) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15003) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB15003) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |