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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | eIF3A rabbit polyclonal |
Gene Name: | Eukaryotic translation initiation factor 3 subunit A |
Synonyms: | Eif3a, EIF3A, EIF3, EIF3S10, P167, TIF32, eIF3-p170, EIF3-theta, p180, p185, Eukaryotic translation initiation factor 3 subunit A, eIF3A |
Immunogen: | Recombinant protein corresponding to Mouse eIF3A |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 162 kDa |
Observed MW. | 162 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kdney, colon, brain, spleen, lung, liver |
Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Images
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Western blot analysis of eif3 (GB11676) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |