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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ring finger and CCCH-type domains 1 |
| Gene Symbol: | RC3H1 |
| Synonyms: | FHL6; IMDSHY; RNF198; ROQUIN; RC3H1 |
| Gene ID: | 149041 |
| UniProt ID: | Q5TC82 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human RC3H1 (NP_742068.1). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.14 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RC3H1 antibody |
Background
This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from SH-SY5Y cells, using RC3H1 Rabbit pAb (A15569) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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