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HRP-conjugated Goat anti-Mouse IgG (H+L) (100 μl)
| Applications: | WB, DB, ELISA |
| Host Species: | Goat |
| Isotype: | Horseradish peroxidase conjugated IgG |
| Conjugation: | HRP |
| Immunogen: | Mouse IgG |
| Dilution: | WB 1:2000-1:10000; DB 1:2000-1:10000; ELISA 1:5000-1:10000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.13 mg/mL |
| Buffer: | PBS with 0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (AS003) at 1:5000/1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
