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HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb (100 μl)
| Applications: | ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | HRP |
| Gene Name: | immunoglobulin heavy constant gamma 2B |
| Gene Symbol: | Ighg2b |
| Synonyms: | IgG2b; Igh-3; gamma2b |
| Gene ID: | 16016 |
| UniProt ID: | P01867-2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 95-113 of mouse IgG2b (P01867-2) . |
| Dilution: | ELISA 1:5000-1:10000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.13mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgD、Rat IgG、Human IgG. |
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