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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ubiquitin specific peptidase 47 |
| Gene Symbol: | USP47 |
| Synonyms: | TRFP; USP47 |
| Gene ID: | 55031 |
| UniProt ID: | Q96K76 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1128-1287 of human USP47 (NP_060414.3). |
| Dilution: | WB 1:200-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.01 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-USP47 antibody |
Background
Enables WD40-repeat domain binding activity and thiol-dependent deubiquitinase. Involved in several processes, including monoubiquitinated protein deubiquitination; negative regulation of G2/M transition of mitotic cell cycle; and negative regulation of nitrogen compound metabolic process. Located in cytoplasm. Part of SCF ubiquitin ligase complex.
Images
 | Western blot analysis of various lysates using USP47 Rabbit pAb (A15461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP47 Rabbit pAb (A15461) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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