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Biotin Rabbit anti-Mouse CD8a mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | Biotin |
| Gene Name: | CD8 subunit alpha |
| Gene Symbol: | Cd8a |
| Synonyms: | Ly-2; Ly-B; Ly-35; Lyt-2 |
| Gene ID: | 12525 |
| UniProt ID: | P01731 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-196 of mouse CD8a (NP_001074579.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd8a antibody |
Background
Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).
Images
 | Flow cytometry: 1×10^6 RAW264.7 cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with Biotin Rabbit anti-Mouse CD8a mAb (A27373,5 μl/Test,orange line) or Biotin Rabbit IgG isotype control (A25626,5 μl/Test,blue line), followed by APC Streptavidin staining. Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with Biotin Rabbit IgG isotype control (A25626,5 μl/Test,left) or Biotin Rabbit anti-Mouse CD8a mAb (A27373,5 μl/Test,right), followed by APC Streptavidin staining. |
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