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Carbonic Anhydrase 1 (CA1) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | carbonic anhydrase 1 |
| Gene Symbol: | CA1 |
| Synonyms: | CAB; CA-I; Car1; HEL-S-11; Carbonic Anhydrase 1 (CA1) |
| Gene ID: | 759 |
| UniProt ID: | P00915 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) (NP_001122303.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.08 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CA1 antibody |
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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