Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A13682
Availability: In Stock

$108.00

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Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, IHC, IF/IC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Polyclonal antibody
Gene Name:	carbonic anhydrase 9
Gene Symbol:	CA9
Synonyms:	MN; CAIX; Carbonic Anhydrase 9 (CA9/G250)
Gene ID:	768
UniProt ID:	Q16790
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 52-151 of human Carbonic Anhydrase 9 (CA9/G250) (NP_001207.2).
Dilution:	WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method:	Affinity purification
Concentration:	1.02 mg/ml
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.
Documents:	Manual-CA9 antibody

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Images

	Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (A13682) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry analysis of paraffin-embedded Human stomach using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (A13682) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (A13682) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
	Immunofluorescence analysis of HeLa cells using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (A13682) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.