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| Reactivity: | Human, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cellular communication network factor 2 |
| Gene Symbol: | CCN2 |
| Synonyms: | CTGF; NOV2; HCS24; IGFBP8 |
| Gene ID: | 1490 |
| UniProt ID: | P29279 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CTGF (NP_001892.2). |
| Dilution: | WB 1:500-1:10000; IF/IC 1:20-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.74 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CCN2 antibody |
Background
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Images
 | Western blot analysis of lysates from Hep G2 cells using CTGF Rabbit pAb (A11067) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunofluorescence analysis of THP-1 cells using CTGF Rabbit pAb (A11067) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of paraffin-embedded Rat thymus using CTGF Rabbit pAb (A11067) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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