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Reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | CD34 molecule |
Gene Symbol: | CD34 |
Synonyms: | CD34; CD34 molecule; GIG3; MORT1 |
Gene ID: | 947 |
UniProt ID: | P28906 |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 32-290 of human CD34 (NP_001020280.1). |
Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 1.86 mg/ml |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-CD34 antibody |
Background
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Images
![]() | Western blot analysis of various lysates using CD34 Rabbit pAb (A13929) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human placenta using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunofluorescence analysis of TF-1 cells using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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