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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | CD38 molecule |
| Gene Symbol: | CD38 |
| Synonyms: | ADPRC1; cADPR1; ADPRC 1; CD38 |
| Gene ID: | 952 |
| UniProt ID: | P28907 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 43-300 of human CD38 (NP_001766.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:2000-1:4000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.42 mg/mL |
| Buffer: | PBS with 0.09% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CD38 antibody |
Background
The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using CD38 Rabbit pAb (A13611) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Negative control (NC): Hep G2. Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD38 Rabbit pAb (A13611) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunofluorescence analysis of rat spleen cells using CD38 Rabbit pAb (A13611) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of THP-1 cells using CD38 Rabbit pAb (A13611) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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