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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | cell division cycle associated 8 |
| Gene Symbol: | CDCA8 |
| Synonyms: | BOR; DasraB; MESRGP; BOREALIN; CDCA8 |
| Gene ID: | 55143 |
| UniProt ID: | Q53HL2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CDCA8 (NP_060571.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.27 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CDCA8 antibody |
Background
This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.
Images
 | Western blot analysis of lysates from Mouse testis, using CDCA8 Rabbit pAb (A12594) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CDCA8 Rabbit pAb (A12594) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using CDCA8 Rabbit pAb (A12594) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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