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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cadherin 1 |
| Gene Symbol: | CDH1 |
| Synonyms: | UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; E-Cadherin |
| Gene ID: | 999 |
| UniProt ID: | P12830 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 373-622 of human E-Cadherin (NP_004351.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.99 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CDH1 antibody |
Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
Images
 | Western blot analysis of lysates from HCT116 cells, using E-Cadherin Rabbit pAb (A11492) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from Mouse liver using E-Cadherin Rabbit pAb (A11492) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using E-Cadherin Rabbit pAb (A11492) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunofluorescence analysis of A-431 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of MCF7 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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