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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | cyclin dependent kinase 12 |
| Gene Symbol: | CDK12 |
| Synonyms: | CRK7; CRKR; CRKRS; CDK12 |
| Gene ID: | 51755 |
| UniProt ID: | Q9NYV4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK12 (NP_057591.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 2.79 mg/ml |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CDK12 antibody |
Background
Enables RNA polymerase II CTD heptapeptide repeat kinase activity and cyclin binding activity. Involved in phosphorylation of RNA polymerase II C-terminal domain; protein autophosphorylation; and regulation of MAP kinase activity. Located in nuclear speck. Part of cyclin K-CDK12 complex.
Images
 | Western blot analysis of lysates from LNCaP cells, using CDK12 Rabbit pAb (A14894) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from Mouse brain, using CDK12 Rabbit pAb (A14894) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CDK12 Rabbit pAb (A14894) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus using CDK12 Rabbit pAb (A14894) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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