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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG2a,kappa |
| Clonality: | Polyclonal antibody |
| Gene Name: | cyclin dependent kinase inhibitor 1B |
| Gene Symbol: | CDKN1B |
| Synonyms: | KIP1; MEN4; CDKN4; MEN1B; P27KIP1; CDKN1B/p27KIP1 |
| Gene ID: | 1027 |
| UniProt ID: | P46527 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:20-1:50 |
| Purification Method: | Affinity purification |
| Concentration: | 0.53mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CDKN1B antibody |
Background
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Images
 | Western blot analysis of various lysates using CDKN1B/p27KIP1 Rabbit pAb (A0290) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using CDKN1B/p27KIP1 Rabbit pAb (A0290) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat liver using CDKN1B/p27KIP1 Rabbit pAb (A0290) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using CDKN1B/p27KIP1 Rabbit pAb (A0290) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using CDKN1B/p27KIP1 Rabbit pAb (A0290) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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