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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | centromere protein E |
| Gene Symbol: | CENPE |
| Synonyms: | KIF10; CENP-E; MCPH13; PPP1R61; CENPE |
| Gene ID: | 1062 |
| UniProt ID: | Q02224 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2542-2701 of human CENPE (NP_001804.2). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.92 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CENPE antibody |
Background
Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
Images
 | Western blot analysis of various lysates using CENPE Rabbit pAb (A15263) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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