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| Reactivity: | Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | collagen type X alpha 1 chain |
| Gene Symbol: | COL10A1 |
| Synonyms: | COL10A1; collagen alpha-1(X) chain; Collagen X/COL10A1 |
| Gene ID: | 1300 |
| UniProt ID: | Q03692 |
| Clone ID: | 0F5Q9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen X/COL10A1 (Q03692). |
| Dilution: | WB 1:1000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-COL10A1 antibody |
Background
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
Images
 | Western blot analysis of various lysates using Collagen X/COL10A1 Rabbit mAb (A11645) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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