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Anti-CORO1B Rabbit pAb (100 μl)
| Reactivity: | M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Coronin-1B |
Synonyms: | Coronin-2 , Coronin 1B, Coronin actin binding protein 1B |
Immunogen: | Recombinant protein corresponding to Mouse CORO1B |
Uniprot ID: | Q9WUM3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 54 kDa / 55 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | kidney |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction (By similarity).
Images
| WB analysis of CORO1B(GB114133). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of CORO1B(GB114133)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |