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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | caspase 8 |
| Gene Symbol: | Casp8 |
| Synonyms: | MACH; Mch5; FLICE; CASP-8; Caspase-8 |
| Gene ID: | 12370 |
| UniProt ID: | O89110 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 4-480 of mouse Caspase 8 (NP_033942.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.49 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Casp8 antibody |
Background
This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Images
 | Western blot analysis of lysates from Mouse liver, using Caspase-8 Rabbit pAb (A11324) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from Mouse thymus, using Caspase-8 Rabbit pAb (A11324) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit pAb (A11324) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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