Key Features: | • Superior transfection efficiency in both suspension and adherent CHO cells • Does not require removal of serum or culture medium • Does not require washing or changing of medium after transfection • Low cytotoxicity |
Description: | PrimeFect™ Transfection Reagent for CHO Cells is a biodegradable polymer based transfection reagent that forms a complex with DNA, and transports the complex into a variety of adherent and suspension cell lines. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis, leading to much less cytotoxicity. |
Component: | 1. PrimeFect™ Transfection Reagent for CHO Cells: 1 ml |
Storage Condition: | Store at 4°C. If stored properly, the product is stable for 12 months or longer. |
Documents: | Manual |
Case Study
Comparison of PrimeFect™ for CHO and Lipofectamine® 2000 transfection efficiencies using GFP visualization
CHO cells were transfected with a GFP-expressing plasmid using either PrimeFect™ transfection reagent for CHO cells (left), or Lipofectamine® 2000 (right). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).