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Anti-Collagen III Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Collagen alpha-1(III) chain |
Synonyms: | Col3a1, EDS4A, type III preprocollagen alpha 1 chain, Type III collagen, Collagen III alpha 1 chain precursor , Collagen III alpha 1 polypeptide |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human collagen Ⅲ |
Uniprot ID: | P02461 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 138 kDa / 140-180 kDa , 200 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:500-1:1000 | skin, lung, heart |
Background
Type III collagen is a fibrillar forming collagen comprising three a1(III) chains and is expressed in early embryos and throughout embryogenesis. In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen. COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with type III and IV Ehlers-Danlos syndrome, and with aortic and arterial aneurysms.
Images
| WB analysis of Collagen III(GB114121). Sample: Protein treated by RIPA Lysis Buffer (G2002),☆ sample of skin treated by RIPA+SDS Lysis Buffer. Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |