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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | DDB1 and CUL4 associated factor 4 |
| Gene Symbol: | DCAF4 |
| Synonyms: | WDR21; WDR21A; DCAF4 |
| Gene ID: | 26094 |
| UniProt ID: | Q8WV16 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DCAF4 (NP_056419.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.94 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-DCAF4 antibody |
Background
This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene.
Images
 | Western blot analysis of lysates from U-251MG cells, using DCAF4 Rabbit pAb (A14869) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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