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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | dimethylarginine dimethylaminohydrolase 1 |
| Gene Symbol: | DDAH1 |
| Synonyms: | DDAH; DDAHI; DDAH-1; HEL-S-16; DDAH1 |
| Gene ID: | 23576 |
| UniProt ID: | O94760 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 3.47 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DDAH1 antibody |
Background
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Images
 | Western blot analysis of various lysates using DDAH1 Rabbit pAb (A10295) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of NIH-3T3 cells using DDAH1 Rabbit pAb (A10295) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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