Dynamin 3 Rabbit pAb (20 μl)

Dynamin 3 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15148
Availability: In Stock
$108.00
-+
Reactivity:   Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   dynamin 3
Gene Symbol:   DNM3
Synonyms:   Dyna III; Dynamin 3
Gene ID:   26052
UniProt ID:   Q9UQ16
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 410-555 of human Dynamin 3 (NP_001265181.1).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:100
Purification Method:   Affinity purification
Concentration:   2.34 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-DNM3 antibody




Background

This gene encodes a member of a family of guanosine triphosphate (GTP)-binding proteins that associate with microtubules and are involved in vesicular transport. The encoded protein functions in the development of megakaryocytes. Alternative splicing results in multiple transcript variants.




Images

DNM3 antibodyWestern blot analysis of various lysates using Dynamin 3 Rabbit pAb (A15148) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of L929 cells using Dynamin 3 Rabbit pAb (A15148) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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