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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | dynein light chain LC8-type 2 |
| Gene Symbol: | DYNLL2 |
| Synonyms: | Dlc2; DNCL1B; RSPH22; DYNLL2 |
| Gene ID: | 140735 |
| UniProt ID: | Q96FJ2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human DYNLL2 (NP_542408.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.58 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-DYNLL2 antibody |
Background
Predicted to enable dynein intermediate chain binding activity and dynein light intermediate chain binding activity. Predicted to be involved in cilium assembly. Located in 9+0 non-motile cilium and centrosome. Is active in glutamatergic synapse and postsynapse.
Images
 | Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of NIH/3T3 cells using DYNLL2 Rabbit pAb (A13888) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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