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| Reactivity: | Human |
| Applications: | WB, IP, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | E2F transcription factor 6 |
| Gene Symbol: | E2F6 |
| Synonyms: | E2F-6; E2F6 |
| Gene ID: | 1876 |
| UniProt ID: | O75461 |
| Clone ID: | 9I5M0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-281 of human E2F6 (NP_937987.2) |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-E2F6 antibody |
Background
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Images
 | CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg E2F6 Rabbit mAb (A11546), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1), as shown in figure. |
 | Western blot analysis of various lysates using E2F6 Rabbit mAb (A11546) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg E2F6 antibody (A11546). Western blot was performed from the immunoprecipitate using E2F6 antibody (A11546) at a dilution of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (A11546) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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