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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 3 subunit B |
| Gene Symbol: | EIF3B |
| Synonyms: | PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116; eIF3B |
| Gene ID: | 8662 |
| UniProt ID: | P55884 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human eIF3B (NP_003742.2). |
| Dilution: | WB 1:1000-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.82 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-EIF3B antibody |
Background
Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in extracellular exosome. Part of eukaryotic translation initiation factor 3 complex.
Images
 | Western blot analysis of various lysates using eIF3B Rabbit pAb (A10259) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using eIF3B Rabbit pAb (A10259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using eIF3B Rabbit pAb (A10259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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