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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 3 subunit H |
| Gene Symbol: | EIF3H |
| Synonyms: | EIF3S3; eIF3-p40; eIF3-gamma; EIF3H |
| Gene ID: | 8667 |
| UniProt ID: | O15372 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.46 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-EIF3H antibody |
Background
Enables deubiquitinase activity. Contributes to translation initiation factor activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process and translational initiation. Located in extracellular exosome and membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in breast cancer; prostate cancer; and prostate carcinoma. Biomarker of prostate cancer.
Images
 | Western blot analysis of various lysates using EIF3H Rabbit pAb (A13378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using EIF3H Rabbit pAb (A13378). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 1 μg EIF3H antibody (A13378). Western blot was performed from the immunoprecipitate using EIF3H antibody (A13378) at a dilution of 1:1000. |
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