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| Reactivity: | Human, Mouse |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | E74 like ETS transcription factor 3 |
| Gene Symbol: | ELF3 |
| Synonyms: | ERT; ESX; EPR-1; ESE-1; ESE1/ELF3 |
| Gene ID: | 1999 |
| UniProt ID: | P78545 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_004424.3). |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.44 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ELF3 antibody |
Background
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm.
Images
 | Western blot analysis of various lysates, using ESE1/ELF3 Rabbit pAb (A13489) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunoprecipitation analysis of 150 μg extracts of A549 cells using 3 μg ESE1/ESE1/ELF3 antibody (A13489). Western blot was performed from the immunoprecipitate using ESE1/ESE1/ELF3 antibody (A13489) at a dilution of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
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