Fibrillarin/U3 RNP Rabbit mAb (20 μl)

Fibrillarin/U3 RNP Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A0850
Availability: In Stock
$148.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, IP, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: fibrillarin
Gene Symbol: FBL
Synonyms: FIB; FLRN; Nop1; RNU3IP1; Fibrillarin/U3 RNP
Gene ID: 2091
UniProt ID: P22087
Clone ID: 9J5O2
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 222-321 of human Fibrillarin/U3 RNP (P22087).
Dilution: WB 1:1000-1:10000; IF/IC 1:100-1:1000
Purification Method: Affinity purification
Concentration: 0.25 mg/mL
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-FBL antibody




Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.




Images

FBL antibodyWestern blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit mAb (A0850) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit mAb (A0850) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Confocal imaging of U-2OS cells using Fibrillarin/U3 RNP Rabbit mAb (A0850,dilution 1:100) (Red) . The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunoprecipitation of Fibrillarin/U3 RNP Rabbit mAb from 300 µg extracts of 293F cells was performed using 3 µg of Fibrillarin/U3 RNP Rabbit mAb (A0850). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Fibrillarin/U3 RNP Rabbit mAb (A0850) at a dilution of 1 : 2000.



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