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| Reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | fibrillarin |
| Gene Symbol: | FBL |
| Synonyms: | FIB; FLRN; Nop1; RNU3IP1; Fibrillarin/U3 RNP |
| Gene ID: | 2091 |
| UniProt ID: | P22087 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human Fibrillarin/U3 RNP (NP_001427.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.68 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FBL antibody |
Background
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Images
 | Western blot analysis of various lysates using Fibrillarin/U3 RNP Rabbit pAb (A13490) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using Fibrillarin/U3 RNP Rabbit pAb (A13490) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using Fibrillarin/U3 RNP Rabbit pAb (A13490) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Fibrillarin/U3 RNP Rabbit pAb (A13490) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg Fibrillarin/U3 RNP antibody (A13490). Western blot was performed from the immunoprecipitate using Fibrillarin/U3 RNP antibody (A13490) at a dilution of 1:1000. |
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