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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | fibronectin 1 |
| Gene Symbol: | FN1 |
| Synonyms: | FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF; Fibronectin |
| Gene ID: | 2335 |
| UniProt ID: | P02751 |
| Clone ID: | 5I8T1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-2477 of human FN1 (P02751). |
| Dilution: | WB 1:2000-1:6000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FN1 antibody |
Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Images
 | Western blot analysis of lysates from Hep G2 cells, using Fibronectin Rabbit mAb (A12977) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Confocal imaging of HeLa cells using Fibronectin Rabbit mAb (A12977,dilution 1:50)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. |
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