ABflo® 405 Mouse anti-Rabbit IgG (Fc) mAb (100 T)

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Images

Flow cytometry: 1×10^6 BeWo cells (negative control,left) and HeLa cells (right) were surface-stained with Rabbit anti-Human CD261/TRAIL-R1 mAb (2 μg/mL,orange line) or ABflo® 405 Rabbit IgG isotype control (5 μl/Test,blue line), then stained with ABflo® 405 Mouse anti-Rabbit IgG (Fc) mAb (A24929,5 μl/Test) was used as a secondary antibody. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1×10^6 HeLa cells were surface-stained with ABflo® 405 Rabbit IgG isotype control (5 μl/Test,left) or Rabbit anti-Human CD261/TRAIL-R1 mAb (2 μg/mL,right). ABflo® 405 Mouse anti-Rabbit IgG (Fc) mAb (A24929,5 μl/Test) was used as a secondary antibody.

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