ABflo® 488 Mouse Anti-Rabbit IgM (Fc) mAb (100 T)

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Images

	Flow cytometry: 1×10^6 RK13 cells (negative control,left) and Rabbit PBMC (right) were surface-stained with ABflo® 488 Mouse Anti-Rabbit IgM (Fc) mAb (A25158,5 μl/Test,orange line) or ABflo® 488 Mouse IgG1 isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1×10^6 Rabbit PBMC were surface-stained with ABflo® 488 Mouse IgG1 isotype control (5 μl/Test,left) or ABflo® 488 Mouse Anti-Rabbit IgM (Fc) mAb (A25158,5 μl/Test,right).

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