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ABflo® 488 Rabbit anti-Human CD104/Integrin β4 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC,FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | integrin subunit beta 4 |
| Gene Symbol: | ITGB4 |
| Synonyms: | CD104; GP150; JEB5A; JEB5B |
| Gene ID: | 3691 |
| UniProt ID: | P16144 |
| Clone ID: | 9R2J4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 611-710AA of human CD104/Integrin β4 (NP_000204.3). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ITGB4 Antibody |
Background
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Images
 | Flow cytometry: 1×10^6 K-562 cells (negative control,left) and A431 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD104/Integrin β4 mAb (A25062,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 A431 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD104/Integrin β4 mAb (A25062,5 μl/Test,right). |
 | Confocal imaging of A-431 cells using ABflo® 488 Rabbit anti-Human CD104/Integrin β4 mAb (A25062, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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