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ABflo® 488 Rabbit anti-Human CD142 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | coagulation factor III, tissue factor |
| Gene Symbol: | F3 |
| Synonyms: | TF; TFA; CD142 |
| Gene ID: | 2152 |
| UniProt ID: | P13726 |
| Clone ID: | 2O0W3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-251 of human CD142 (NP_001984.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-F3 Antibody |
Background
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
Images
 | Immunofluorescence analysis of A-431 cells using ABflo® 488 Rabbit anti-Human CD142 mAb (A22492) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Flow cytometry: 1×10^6 HEL cells (negative control,left) and A-431 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD142 mAb (A22492,2 μg/mL,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,2 μg/mL,blue line). Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry: 1×10^6 A-431 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD142 mAb (A22492,5 μl/Test,right). |
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