ABflo® 488 Rabbit anti-Human CD225/IFITM1 mAb (100 T)

Brand: Abclonal
Cat. #: A22310
Availability: In Stock

$218.00

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Description

Reactivity:	Human
Applications:	IF/ICC, FC (intra)
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 488. Ex:491nm. Em:516nm.
Gene Name:	interferon induced transmembrane protein 1
Gene Symbol:	IFITM1
Synonyms:	9-27; CD225; IFI17; LEU13; DSPA2a
Gene ID:	8519
UniProt ID:	P13164
Clone ID:	9A8O7
Immunogen:	Recombinant fusion protein containing a sequence within amino acids 1-80 of human CD225/IFITM1 (NP_003632.4).
Dilution:	IF/ICC,1:50 - 1:200\|FC (intra),5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.02 mg/mL
Buffer:	PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-IFITM1 Antibody

Background

Interferon-induced transmembrane (IFITM) proteins are a family of interferon induced antiviral proteins. The family contains five members, including IFITM1, IFITM2 and IFITM3 that belong to the CD225 superfamily. The protein encoded by this gene restricts cellular entry by diverse viral pathogens, such as influenza A virus, Ebola virus and Sars-CoV-2.

Images

	Immunofluorescence analysis of K-562 cells using ABflo® 488 Rabbit anti-Human CD225/IFITM1 mAb (A22310) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Flow cytometry: 1×10^6 U-251MG cells (Low Expression,left) and K-562 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human CD225/IFITM1 mAb (A22310,2 μg/mL,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,2 μg/mL,blue line). Non-fluorescently stained cells was used as blank control (red line).
	Flow cytometry: 1×10^6 K-562 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD225/IFITM1 mAb (A22310,5 μl/Test,right).
	Flow cytometry: 1×10^6 U-251MG cells (Low Expression) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD225/IFITM1 mAb (A22310,5 μl/Test,right).