ABflo® 488 Rabbit anti-Human CD24 mAb (100 T)

ABflo® 488 Rabbit anti-Human CD24 mAb (100 T)

Brand: Abclonal
Cat. #: A25380
Availability: In Stock
$178.00
-+
Reactivity: Human    
Applications: IF/ICC, FC    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal Antibody    
Conjugation: ABflo® 488. Ex:491nm. Em:516nm.
Gene Name: CD24 molecule
Gene Symbol: CD24
Synonyms: CD24A
Gene ID: 100133941
UniProt ID: P25063
Clone ID: 3E4M8
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-80 of human CD24 (NP_037362.1).
Dilution: IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume
Purification Method: Affinity purification
Concentration: 0.04 mg/mL
Buffer: PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-CD24 antibody




Background

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants.




Images

CD24 antibodyConfocal imaging of MCF7 cells using ABflo® 488 Rabbit anti-Human CD24 mAb (A25380, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry: 1×10^6 293F cells (Low Expression,left) and MCF7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD24 mAb (A25380,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1×10^6 MCF7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD24 mAb (A25380,5 μl/Test,right).
Flow cytometry: 1×10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 488 Rabbit anti-Human CD24 mAb (A25380,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1×10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD24 mAb (A25380,5 μl/Test,right).



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