ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb (100 T)

Brand: Abclonal
Cat. #: A23801
Availability: In Stock

$218.00

Qty

Description

Reactivity:	Human
Applications:	IF/ICC, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 488. Ex:491nm. Em:516nm.
Gene Name:	CD79b molecule
Gene Symbol:	CD79b
Synonyms:	B29; IGB; AGM6; Igbeta
Gene ID:	974
UniProt ID:	P40259
Clone ID:	10L9A4
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-159 of human CD79b/Igβ(NP_000617.1).
Dilution:	IF/ICC,1:50 - 1:200\|FC,5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.04 mg/mL
Buffer:	PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-CD79b Antibody

Background

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.

Images

	Confocal imaging of Daudi cells using ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb (A23801, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Flow cytometry: 1×10^6 HAP1 cells(negative controll,left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb(A23801,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry:1×10^6 Daudi cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb(A23801,5 μl/Test,right).