ABflo® 488 Rabbit anti-Human Calreticulin mAb (100 T)

Brand: Abclonal
Cat. #: A27387
Availability: In Stock

$530.00

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Description

Reactivity:	Human
Applications:	FC (intra)
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 488. Ex:491nm. Em:516nm.
Gene Name:	calreticulin [ Homo sapiens
Gene Symbol:	CALR
Synonyms:	RO; CRT; SSA; CALR1; cC1qR; HEL-S-99n
Gene ID:	811
UniProt ID:	P27797
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 335-417 of human Calreticulin (NP_004334.1).
Dilution:	FC (intra),5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.04 mg/ml
Buffer:	PBS with 0.09% Sodium azide,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-CALR Antibody

Background

Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.

Images

	Flow cytometry: 1×10^6 knockout (KO) HeLa cells (negative control,left) and HeLa cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human Calreticulin mAb (A27387,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1×10^6 HeLa cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human Calreticulin mAb (A27387,5 μl/Test,right).